Abstract

ABSTRACT:
BACKGROUND:
There is an increasing evidence suggesting that high-risk human papilloma virus (HPV) was involved in cancers other than cervical cancer. A number of reports have identified HPV DNA in breast tissue and breast cancer specimens, suggesting that the virus could play a role in the pathogenesis of this tumor. Therefore,
OBJECTIVE:
was directed towards the use of In situ molecular methods to localize the virus in mammary gland tissue. In addition, this study investigated the prevalence of high-risk HPV18 infections in Iraqi women with and without ductal carcinoma of the breast.
PATIENTS AND METHODS:
Twenty night cases of ductal carcinoma patients and 44 controls obtained from adjacent area to benign breast lesions. Formalin fixed, paraffin embedded specimens were used by In situ hybridization technique for detection of HPV18 subtype. Data analysis was performed by SPSS 20 software using descriptive statistics and Chi-square tests.
RESULTS:
The HPV18 were identified in 65.5% and 20.5% of the ductal carcinoma and control breast tissue specimens respectively. Statistically, the difference between the normal and ductal carcinoma cases were highly significant (P=0.001
CONCLUSION:
Statistically, In situ hybridization revealed a significant increase of HPV18 in cases of ductal carcinoma (DC) (65.5%) when compared to their controls (20.5%) .
Most HPV were localized in the nuclei of integrative form.
HPV18 were detected in skin and mammary tissue of both ductal carcinoma and control cases. This may indicates that HPV18 has a role in ductal carcinoma pathogenisis.