Abstract

ABSTRACT:
BACKGROUND:
Sperm motility is essential in fertilization because non active or mal active sperms never reach the ovum, but the progress in the assisted reproductive techniques makes it easy for doctors to select viable sperm to be used in intracytoplasmic sperm injection thus the differentiation between viable and non-viable sperms is very important especially in sever asthenospermic samples.
OBJECTIVE:
To evaluate low level Helium Neon laser irradiation of the whole seminal sample as a method to activate mal or non-active sperms and to study and follow up the late effect of laser irradiation on the seminal samples.
MATERIALS AND METHODS:
100 samples were included in this study from patients attending seminal fluid examination lab. After digital seminal fluid examination, samples were divided in 2 parts, the first was incubated for 120min at 37C and the other was irradiated with 623.8 wave length Helium Neon laser and an output power of 2mW at 1cm distance for 10min and then examined digitally after irradiation then incubated with the first part to 120min also to be examined again with the first part.
RESULTS:
It was found that there is a significant increase in the progressive motile sperm percentage in the laser irradiated samples from about 33.6% to 41.6% (p<0.005) and also decrease in the percentage of the immotile sperms from about 44.1% to 34.4% (p<0.001). While after 120min incubation, active sperms percentage in the irradiated sample was about 43% which is higher than the non-treated part (31.6%; p<0.01) that showed also higher percentage of immotile sperms (52.8%) against (39.9%) in the treated part.
CONCLUSION:
Helium Neon laser irradiation to the whole seminal sample increase the sperms activity and decrease the percentage of immotile sperms which mean that it stimulates viable but immotile sperm to move. And also, sperms in laser irradiated samples preserved their increased activity for more than 2hours after laser irradiation.