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Interphase Fluorescence in Situ hybridization Analysis of 17p13.1 and 11q22.3 Deletions among CLL Patients

,

Document Type : Research Paper

Abstract

BACKGROUND:
Chronic lymphocytic leukemia (CLL) is a B‐cell neoplasm. It is the most frequent adult leukemia in European countries, comprising up to 30% of all adult leukemias with male predominance. Detection of a 17p13.1 deletion (loss of TP53) or 11q22.3 deletion (loss of ATM), by fluorescence in situ hybridization (FISH), is associated with a poorer prognosis.
OBJECTIVE:
This study aims to analyze the frequency of cytogenetics abnormalities among CLL patients.
PATIENTS, MATERIALS AND METHODS:
This study enrolled 30 adult patients presented with chronic lymphocytic leukemia visiting Baghdad teaching hospital/hematology center lab/cytogenetic unit-Baghdad /Iraq during the period extending from Mar. till Nov. 2020.The patients were diagnosed on the basis of standard clinical (Lymph node involvement and / or hepatosplenomegally), hematological and immunophenotypic criteria for diagnosis of B-CLL. Interphase FISH technique was performed on peripheral Blood samples using XL ATM/TP53 dual-color probe.
RESULTS:
We studied 30 CLL patients by I-FISH technique to detect the occurrence of cytogenetic abnormalities, eighteen (60%) had one or more cytogenetic abnormalities; Eleven (36.7%) had 17p- (including 2 with 17p- and 11q- in separate clones), Five (16.7%) had 11q-, and Two (6.7%) of patients had clonal 17p-/11q-. Twelve (40%) had neither 17p- nor 11q-. There was no statistically significant clinico pathological correlation with these cytogenetic abnormalities; p-value >0.05.
CONCLUSION:
The cytogenetic abnormalities including 17p-, 11q- and clonal 17p-/11q- among CLL patients occur within the reported figures worldwide. There was no statistically significant clinico pathological correlation with these cytogenetic abnormalities.
 

Keywords

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Iraqi Postgraduate Medical Journal
Volume 20, Issue 1
January 2021
Page 39-45
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