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The Role of Calretinin Immunohistochemistry in Detection of Ganglion Cell in Hirschsprung Disease

,

Document Type : Research Paper

Abstract

ABSTRACT:
BACKGROUND:
Hirschsprung’s disease is a developmental abnormality of enteric nervous system characterized by lack of ganglion cell and neural hypertrophy in the Meissner and Auerbach’s plexuses within variable length of the colon, the Calretinin Immunohistochemistry is one of the newer methods overcome difficulties in diagnosis of Hirschsprung’s disease especially immature ganglia in neonates in submucosal plexus.
AIM OF STUDY:
This study was performed to evaluate the diagnostic value of Calretinin immunohistochemistry in                 the Hirschsprung’s Disease diagnosis.
MATERIALS AND METHOD:
A retrospective and prospective case series study at the period from (Jan2018-Jan2020) included a total 50 formalin-fixed paraffin-embedded blocks of distal colonic and rectal incisional biopsies from infant and young children patient who suspected with Hirschsprung’s disease, The study was undertaken in the department of pathology, collage of medicine/ University of Mosul, a clinicopathological data including (age, gender, site, type of surgical procedure) were obtained from patients' files This study was performed by using the primary antibody of Calretinin.
RESULTS:
The study done on 50 cases their age from 1 day to 14 years. The universal sensitivity in this study to diagnose presence or loss of enteric ganglia by calretinin immunostaining was 90% and the specificity is 100% with positive and negative predictive value of 100% and 97 % respectively.
CONCLUSION:
Calretinin marker considered as a reliable, valuable marker in Hirschsprung disease diagnosis and carry high sensitivity and specificity.
 
 

Keywords

References
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Iraqi Postgraduate Medical Journal
Volume 20, Issue 4
October 2021
Page 368-372
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