Authors
Pathology, University of Ibn Sina /College of Medicine., Baghdad, Iraq.
Abstract
BACKGROUND:
Urothelial carcinoma is the most common histological type of bladder cancer, it accounts for 90% of bladder cancers. Annexin A6 (AnxA6) is a member of a superfamily of Ca2+dependent membrane-binding proteins.
AIM OF STUDY:
To study the expression of Annexin A6 in high grade urothelial carcinoma and cystitis using anti_Annexin A6 antibody; and to correlate the results with some clinico-pathological parameters such as age, sex, presence of necrosis, inflammation, hemorrhage and muscle invation.
MATERIAL AND METHODS:
A retrospective case control study included a total of 60 paraffin embedded samples were divided into two groups: 30 cases of high grade urothelial carcinoma and 30 cases of cystitis. The samples were collected from the archives of teaching labs of Al Yarmouk Teaching Hospital and the archives of Almustansiryah College of medicine for a period from November 2019 to November 2020.
RESULTS:
Annexin A6 was positive in 73.3% of urothelial carcinoma cases. Males were 86.3% while female were 13.6% of positive cases. Annexin A6 was positive in 40% in cystitis samples.
CONCLUSION:
Annexin A6 expression was significant in high grade urothelial carcinoma samples as compared with cystitis samples. In urothelial carcinoma samples, Annexin A6 was significantly associated with the presence of inflamation, hemorrhage, necrosis and muscle invasion, but there was no association with other clinicopathological parameters. Annexin A6 expression shows significant association in primary tumors rather than recurrent tumors.
- Brausi, M., Witjes, J.A., Lamm, D. et al, A review of current guidelines and best practice recommendations for the management of nonmuscle invasive bladder cancer by the International Bladder Cancer Group. J Urol. 2011;186:2158–67.
- Iraq Cancer Board, results of Iraqi Cancer Registry (1994-2015), Ministry of Health, Iraq Cancer Registry Centre, Baghdad – Iraq, 2015.
- Cronin, K.A., Lake, A.J., Scott, S., Sherman, R.L., Noone, A.M., Howlader, N., Henley, S.J., Anderson, R.N., Firth, A.U., Ma, J. and Kohler, B.A.,. Annual Report to the Nation on the Status of Cancer, part I: National cancer statistics. Cancer, 2018;124:.2785-2800.
- Bharucha AE, Trabuco E. Functional and chronic anorectal and pelvic pain disorders. Gastroenterol Clin North Am. 2008 ;37:685-96.
- Crompton MR, Owens RJ, Totty NF, Moss SE, Waterfield MD, Crumpton MJ (Jan 1988). "Primary structure of the human, membrane-associated Ca2+-binding protein p68 a novel member of aprotein family". The EMBO Journal. 7 (1):217.
- Monastyrskaya K, et alJ.Urol.Med.2013;190:325-33.
- Roupret M, Babjuk M, Comperat E, et al. European Association of Urology Guidelines on Upper Urinary Tract Urothelial Carcinoma: 2017 Update. Eur Urol. 2018;73:111-22.
- MOHAMMED, M.J. and AHMED, B.S., 2019. EXPRESSION OF CANCER STEM CELL MARKER OCTAMER-BINDING TRANSCRIPTION FACTOR 4 IN HIGH-GRADE TRANSITIONAL CELL CARCINOMA. Asian J Pharm Clin Res, 12(6),pp.313-316.
- Koyuncuer A. Histopathologic Evolution of Urothelial Carcinoma Recurrence in Transurethral Resection of the Urinary Bladder:35 Consecutive Cases And Literature Review. Asian Pac J Cancer Prev. 2017;18(2):459-463.
- Shen Z, Xie L, Chen T, et al. Risk factors predictive of recurrence and progression for patients who suffered initial recurrence after transurethral resection of stage pT1 bladder tumor in Chinese population: A retrospective study. Medicine. 2016;95: e2625.
- Mohammed-Najeeb, A.M., Ahmed, B.S., Lefta, H., Al-Kabbi, M.M. Immunohistochemical expression of bak and p27 proteins in low and high grade urothelial bladder carcinoma in correlation with some clinico-pathological parameters. Biochemical and cellular Archies, 2020, 20, pp. 3319-3325.
- Aziz, H.M., Ahmed, B.S., Mughir Al-Doghan, I.E. IMMUNOHISTOCHEMICAL EXPRESSION OF ANNEXIN A6 PROTEINS IN BREAST DUCTAL CARCINOMA IN CORRELATION WITH SOME CLINICOPATHOLOGICAL PARAMETERS IN A SAMPLE OF IRAQI PATIENTS. Biochemical and Cellular Archives, 2020;20:4687-92.
- Noreen, S., Gardner, Q. A., Fatima, I., Sadaf, S., Akhtar, M. W., Upregulated Expression of Calcium‐Dependent Annexin A6: A Potential Biomarker of Ovarian Carcinoma. Prot. Clin. Appl. 2020;14:1900078.
- Monastyrskaya K, Babiychuk EB, Draeger A, Burkhard FC. Down-regulation of annexin A1 in the urothelium decreases cell survival after bacterial toxin exposure. J Urol. 2013;190:325-33.
- Langbein S, Lehmann J, Harder A, et al. Protein profiling of bladder cancer using the 2D-PAGE and SELDI-TOF-MS technique. Technology in Cancer Research & Treatment. 2006;5:67-72.
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