,
Document Type : Research Paper
Abstract
Colorectal cancer is the most common gastrointestinal tract cancer worldwide. Cyclin D1 shows increased expression both in advanced adenomas , high dysplasia grade, and/or villous morphology) and colorectal adenocarcinoma with loss of expression in normal cells.
Aim of study :
To evaluate the IHC expression of Cyclin D1 in colorectal adenoma and compare it to adenocarcinoma and to correlate it with different parameters .
Materials and methods:
This retrospective study was done on tissue blocks of (40) cases (20) of which are colorectal adenomatous polyps,(20) were colorectal adenocarcinoma (covering the period from November 2018 to March 2020).
Results:
Cyclin D1 expression was lost in normal mucosa and showed variable staining, the adenoma staining percentage was score 0 in 20%(4), score I 20%(4) , score II 40%(8) and score III 20%(4) ,none had score IV While the staining percentage of colorectal adenocarcinoma was score 0 10%(2), score I 35%(7) , score II 35%(7), score III 5%(1) and score IV 15%(3) of cases.
The mean immunohistochemical staining score was significantly higher in distal colorectal than proximal adenoma (p=0.045).When adenoma cases were evaluated separately for (histopathological type ,grade, size) 2 cases (10%) had villous adenoma morphology and 9 (45%) for tubular and tubulovillous ,11 (55%) showed high grade dysplasia and 9 (45%) showed low grade dysplasia and colorectal adenoma sizes ranged between (0.3-2.5) cm 15 (75%) were less than 2 cm ,5 (25%) were equal to or larger than 2 cm.
The mean score showed a significant difference according to histopathological type (P value =0.11), grade of dysplasia (P value <0.001) and size ( P value =0.045)in colorectal adenoma.
Conclusion:
There was no significant difference in the Cyclin D1 score in colorectal adenoma and adenocarcinoma and showed a significant difference according to histopathological type , grade of dysplasia and size in colorectal adenoma.
- Rosidah a, norahmawati e, anita kw, al rasyid h. perbedaan ekspresi protein cyclin d1 antara adenoma dan adenokarsinoma kolorektal, serta korelasinya dengan grading dan stadium tumor. majalah kesehatan fkub. the differences in cyclin d1 protein expression between adenoma and colorectal adenocarsinoma, and its correlation with grading and tumor stadium. 2020 oct 5;7(3):149-58.
- Sheref M. El Taher , Risk factors of colorectal cancer;a case-control study, Egypt. J. Community Med. 2016; 34 (1): 1-15.
- Albasri A, Elkablawy M, Ansari I, Alhujaily A. Prognostic Significance of Cyclin D1 Over-expression in Colorectal Cancer: An Experience from Madinah, Saudi Arabia. Asian Pacific Journal of Cancer Prevention. 2019;20(8):2471- 76.
- Murphy G, Devesa S, Cross A, Inskip P, McGlynn K, Cook M. Sex disparities in colorectal cancer incidence by anatomic subsite, race and age. International Journal of Cancer. 2010;128(7):1668-75.
- Gao R, Neutel C, Wai E. Gender differences in colorectal cancer incidence, mortality, hospitalizations and surgical procedures in Canada. Journal of Public Health. 2008;30(2):194-201.
- Abdulwahab Mirza S, Hassan AL-Tai TH, Shaker HH. Immunohitochemical expression of p53 in human colocrectal carcinoma. Journal of the Faculty of Medicine Baghdad. 2016 Oct 2;58(3):289–92.
- Ayerden d. kolon adenom ve adenokarsinomlarinda histopatolojik bulgular ile siklin d1 ve ki-67 ekspresyonunun değerlendirilmesi evaluation of histopathological findings and cyclin d1 and ki-67 expression in adenoma and adenocarcinomas 2017.
- Assessment of immunohistochemical expression of her2\neu and BRCA1 in colorectal tumors and it’s correlation with clinicopathological variables. University of Thi-Qar Journal of Medicine. 2020.
- Bahnassy AA, Zekri AR, El-Houssini S, El-Shehaby AM, Mahmoud MR, Abdallah S, El-Serafi M. Cyclin A and cyclin D1 as significant prognostic markers in colorectal cancer patients. BMC gastroenterology. 2004 ;4(1):22.
- Jiang J, Wang J, Suzuki S, Gajalakshmi V, Kuriki K, Zhao Y et al. Elevated risk of colorectal cancer associated with the AA genotype of the cyclin D1 A870G polymorphism in an Indian population. Journal of Cancer Research and Clinical Oncology. 2005;132(3):193-99.145
- Li Y, Wei J, Xu C, Zhao Z, You T. Prognostic Significance of Cyclin D1 Expression in Colorectal Cancer: A Meta-Analysis of Observational Studies. PLoS ONE. 2014;9(4):e94508.
- Arber N, Hibshoosh H, Moss S, Sutter T, Zhang Y, Begg M et al. Increased expression of cyclin D1 is an early event in multistage colorectal carcinogenesis. Gastroenterology. 1996;110(3):669-74.
- Ogino S, Nosho K, Irahara N, Kure S, Shima K, Baba Y et al. A Cohort Study of Cyclin D1 Expression and Prognosis in 602 Colon Cancer Cases. Clinical Cancer Research. 2009;15(13):4431-38.
- Izawa H, Yamamoto H, Ikeda M, Ikeda K, Fukunaga H, Yasui M et al. Analysis of cyclin D1 and CDK expression in colonic polyps containing neoplastic foci: A study of proteins extracted from paraffin sections. Oncology Reports. 2002.
- Ayhan S, Isisag A, Saruc M, Nese N, Demir MA, Kucukmetin NT. The role of pRB, p16 and cyclin D1 in colonic carcinogenesis. Hepatogastroenterology. 2010;57(98):251-56. PMID: 20583423.
- Jang K, Kim Y, Bae J, Chung M, Moon W, Kang M et al. Expression of Cyclin D1 Is Associated with β-Catenin Expression and Correlates with Good Prognosis in Colorectal Adenocarcinoma. Translational Oncology. 2012;5(5):370-78.
- Marcolino T, Pimenta C, Artigiani Neto R, Castelo P, Silva M, Forones N et al. p53, Cyclin-D1, β-catenin, APC and c-myc in Tumor Tissue from Colorectal and Gastric Cancer Patients with Suspected Lynch Syndrome by the Bethesda Criteria. Asian Pacific Journal of Cancer Prevention. 2020;21(2):343-48.
- Utsunomiya T, Doki Y, Takemoto H, Shiozaki H, Yano M, Sekimoto M et al. Correlation of Beta-Catenin and Cyclin D1 Expression in Colon Cancers. Oncology. 2001;61(3):226-33.
- Nassrat F, Qasim B ,Ali H. Immunohistochemical expression of cyclin D1 in colorectal adenomas: a clinicopathological study. Kasr Al Ainy Medical Journal. 2016;22(3):115.
- Hunter T, Pines J. Cyclins and cancer. II: cyclin D and CDK inhibitors come of age. Cell 1994;79:573–82.
- Zhang T, Nanney LB, Luongo C, Lamps L, Heppner KJ, DuBois RN et al. Concurrent overexpression of cyclin D1 and cyclin-dependent kinase 4 (Cdk4) in intestinal adenomas from multiple intestinal neoplasia (Min) mice and human familial adenomatous polyposis patients. Cancer Res 1997;57:169–75.
- Abdulhameed T, Jaff Z, Maaruf N. Clinicopathological study and immunohistochemical evaluation of cyclin D1 in adenomatous polyps. Zanco Journal of Medical Sciences. 2021;25(1):456–63.
- Su T, Washington M, Ness R, Rex D, Smalley W, Ulbright T et al. Comparison of biomarker expression between proximal and distal colorectal adenomas: The References 85 Tennessee-Indiana Adenoma Recurrence Study. Molecular Carcinogenesis. 2016;56(2):761-73.
)
