More than half of all humans are colonized in their stomachs by Helicobacter pylori . That carriage was nearly universal among adults in developing countries suggests that in earlier times most humans carried these organisms.H. pylori was an important human pathogen that causes gastritis and is strongly associated with gastric ulcers, gastric adenocarcinomas, and mucosa-associated lymphoid tissue lymphomas. In response to H. pylori, interleukin-8 (IL-8) was secreted from host cells to attract components of the innate and adaptive immune systems to the site of infection.CagA+ cells induce higher levels of the proinflammatory IL-8 cytokine. That such effects are specific for CagA island genes had been shown by our study.
To determine the role of incidence of H.pylori CagA positive strain in induce higher level of the proinflammatory cytokine IL-8.
One hundred and fifty(150) Patients attended the Endoscopic Unit at"Gastroenterology and Hepatology Teaching Hospital/ Baghdad Medical City"were included in this study with ages range from 18 years to 65 years . The source of specimens to undergo oesophageal gastroduodenoscopy (OGD) collected from April 2009 to end of March 2010 were eligible for this study.Two types biopsy of samples for histopathology to detect H.pylori and detection of CagA gene by In Situ hybridization(ISH) and blood samples for estimation of serum IL-8 level by ELISA.
There was a significant increase in IL-8 serum levels that P<0.05 due to the significant increase of Cag A in H.pylori positive strains.
Our study revealed that increased in CagA expression in H.pylori positive strains and induced higher levels of the proinflammatory cytokines