Abstract

ABSTRACT:
BACKGROUND:
It is well realized that breast cancer is the most terrifying cancer for females, the commonest malignancy and the second leading cause of cancer death in women. Recent studies suggested that the association of viral infection with breast cancer pathogenesis such as Epstein Barr virus and mouse mammary tumor virus. In addition, human papilloma virus DNA sequences have been isolated. There is relationship between human papilloma virus and other neoplasms (anogenital, skin and 99.7% of cervicouterine carcinomas).
OBJECTIVE:
This study has been undertaken for detecting high risk group HPV genotypes in breast carcinoma in different provinces in Iraq depending on molecular technique.
PATIENTS AND METHODS:
A total of 59 patients include both sexes (male and female) diagnosed histopathologically as having breast cancer were included and studied during the period from January to August, 2010 in different regions in Iraq. In addition to 20 cases of benign breast lesion used as a control group. Theses samples were represented by formalin-fixed paraffin embedded blocks and fresh frozen tissue. A multiplex PCR analysis were done for the DNA extracted from these samples.
RESULT:
The mean age in this study was 45.5 years. Ninety three percentage were female and the remaining were male. Fourty percentage of tumor present in the right side, 59.3% removed by lumpectomy. Eighty six percentage were found to have infiltrating ductal carcinoma followed by infiltrating lobular carcinoma (13.8%). Twelve HPV genotypes were used in this study which of high risk group including (16,13,33,35,18,45,39,59,52,56,58 and 66). HPV type 39,59 and 52 were demonstrated among those patients and the result show about 16.9% of our patients (10 out of 59) had positive HPV demonstrated by PCR in their breast tissue and 83.1% (49 out of 59) were negative for HPV. All the benign breast cases were negative for HPV except one case of fibrocystic disease which was positive for HPV type 39 which was not significant (p value ˃0.05).
CONCLUSION:
The study concluded that high risk group Human Papiloma virus genotypes 39, 59 and 52 genotype were present in breast cancer tissue in Iraqi patients