Abstract

ABSTRACT:
BACKGROUND:
Type 1 diabetes mellitus is a chronic autoimmune disease that involves destruction of the pancreatic beta cells. It is well known that both genetic and environmental factors involved in pathogenesis of type-1diabetes mellitus.
OBJECTIVE:
This study seeks to determine whether there is any association between human leukocyte antigen class I and II alleles and type-1diabetes mellitus.
SUBJECTS AND METHODS:
Seventy type-1 diabetes mellitus patients compared to 30 apparently healthy individual were enrolled in this study. Human leukocyte antigens genotyping were analyzed by polymerase chain reaction sequence specific oligonucleotide technique.
RESULTS:
The present study revealed significantly high frequency of DQB1*0101 and DQB1*0201 alleles among patients in comparison with healthy control, while there was significantly low frequency of each HLA-A*3301, B*0826, DRB1*0701, *1101 and HLA-DQB1*0604 alleles in patients as compared to healthy controls.
CONCLUSION:
These findings indicate that higher frequency of HLA-DQB1*0101 and *0201 alleles may be a risk factor for type-1diabetes mellitus, meanwhile low frequency of HLA-A*3301, B*0826, DRB1*0701, *1101 and HLA-DQB1-*0604 alleles could be a protective factor.